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Hot
Paper in Biotechnology
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"Quantitative analysis of complex
protein mixtures using isotope-coded affinity tags," by S.P. Gygi and
5 others, Nature Biotechnology, 17(10):994-9, October 1999.
[Authors' affiliation: University of
Washington, Seattle]
Abstract: "We describe an
approach for the accurate quantification and concurrent sequence
identification of the individual proteins within complex mixtures. The method
is based on a class of new chemical reagents termed isotope-coded affinity
tags (ICATs) and tandem mass spectroscopy. Using this strategy, we compared
protein expression in the yeast Saccharomyces cerevisiae, using either
ethanol or galactose as a carbon source. The measured differences in protein
expression correlated with known yeast metabolic function under
glucose-repressed conditions. The method is redundant if multiple cysteinyl
residues are present, and the relative quantification is highly accurate
because it is based on stable isotope dilution techniques. The ICAT approach
should provide a widely applicable means to compare quantitatively global
protein expression in cells and tissues."
This 1999 report from Nature Biotechnology
was cited 19 times in current journal articles indexed in the
ISI database during September-October 2001. No other article indexed in the
ISI category of Biotechnology & Applied Microbiology attracted as many
citations during that two-month period. Prior to the most recent bimonthly
count, citations to the paper have accrued as follows:
July-August 2001: 17 citations
May-June 2001: 17
March-April 2001: 17
January-February 2001: 5
November-December 2000: 6
September-October 2000: 15
July-August 2000: 6
May-June 2000: 12
March-April 2000: 2
Total citations to date: 116
SOURCE: Hot
Papers Database (Available from the ISI
Research Services Group in a CD-ROM version containing data on
hundreds of highly cited papers published during the last two years.
User interface permits searching by author, organization, journal,
field, and more. Total citations, as well as citations accrued during
successive bimonthly periods, can be assessed and graphed. Database is
combined with subscription to the ISI newsletter Science
Watch®; updated discs containing the
most recent bimonthly data are mailed with each new issue, six times a
year.)
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